![]() Here's the ingredients label for a bottle of Jose Cuervo Classic Margarita Mix (Non-Alcoholic) :ĭepending on which version you buy, it may have high fructose corn syrup (HFCS) or sucralose instead. Now that you know what's supposed to go in a margarita, let's take a look at a popular store-bought Margarita Mix. To make it simple, it's mostly tequila and citrus with a bit of sugar and depending on the person's preference, salt on the rim. There are lots of ways to make a margarita, but a classic version generally has tequila, lime juice, an orange liqueur (like triple sec, Cointreau or Grand Marnier) or orange juice, and a sweetener like simple syrup to balance out the lime's tartness. Unfortunately, these margarita mixes are filled with crappy, fake ingredients. ![]() But I like real juices and mixers in my drinks, as should most people. Cause those mixes are d-i-s-g-u-s-t-i-n-g. :) Second, I know WAY too many people out there are still drinking pre-made mixes and this makes me very sad. I experimented with many cocktails and martinis during my college years. Lime Wedges – A must for garnish, and also to help the salt stick to the rim.I am SO excited to share this margarita recipe with you guys! Know why? First, I concocted it several years ago when I was living in KC with my sister and attending UMKC.Coarse sea salt works very nicely, but any kind you have is fine! Sea Salt – For salting the rim of your margarita glasses.And unlike stevia that I used to use, Besti doesn’t have that stevia aftertaste. I have tried making this drink with erythritol before, which works okay, but doesn’t dissolve very well. Sweetener – Most skinny margarita recipes are really just their regular counterparts in disguise, using agave nectar… which is still sugar! I use Besti Powdered Monk Fruit Allulose Blend in mine and it tastes the same without all the sugar! Unlike other monk fruit sweeteners that contain erythritol, this one dissolves easily in cold liquids. ![]() FYI, Triple Sec is an orange liqueur and contains 11 grams of sugar per ounce.
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![]() Monza, Silverstone, Imola, Mugello, Spa, Brands Hatch and many more tracks, including the historical reproduction of Monza, that brings again to life the legendary Sopraelevata, the high-speed oval where races were staged until 1961. The circuits have been developed using Laser scan technology, in order to guarantee the highest level of accuracy possible in reproducing real world motor racing environments. Extremely detailed with single player and multiplayer options, exclusive licensed cars reproduced with the best accuracy possible, thanks to the official cooperation of Car Manufacturers.ĪSSETTO CORSA has been developed at the KUNOS Simulazioni R&D office, located just inside the international racing circuit of Vallelunga, allowing the team to develop the game with the cooperation of real world racing drivers and racing teams. The advanced physics engine is being designed to provide a very realistic driving experience, including features and aspects of real cars, never seen on any other racing simulator such as tyre flat spots, heat cycles including graining and blistering, very advanced aerodynamic simulation with active movable aerodynamics parts controlled in real time by telemetry input channels, hybrid systems with kers and energy recovery simulation. Assetto Corsa features an advanced DirectX 11 graphics engine that recreates an immersive environment, dynamic lighthing and realistic materials and surfaces. The psychiatric unit is an example of a subpart that could have its own NPI if the hospital determines that it should. (1) The psychiatric unit in a hospital is not a legal entity but is part of the hospital (the "parent"), which is a legal entity. Here are three examples of organization health care providers that may be considered subparts and may apply for NPIs if so directed by their "parents": Many organization health care providers who apply for NPIs are not legal entities themselves but are parts of other organization health care providers that are legal entities (the "parents"). The Parent Organization LBN and TIN fields can only be completed if the answer to the subpart question is Yes. If the organization is a subpart =, the Parent Organization Legal Business Name (LBN) and Parent Organization Taxpayer Identification Number (TIN) fields must be completed. The "Is the organization a subpart?" question must be answered. 2 = (Non-person): entity other than an individual human being that furnishes health care (for example, hospital, SNF, hospital subunit, pharmacy, or HMO).1 = (Person): individual human being who furnishes health care. ![]() ![]() Full Replica of the CMS (NPPES) NPI Record Field Nameġ0-position all-numeric identification number assigned by the NPS to uniquely identify a health care provider.Ĭode describing the type of health care provider that is being assigned an NPI. This thank-yousir to Glastron dealers and their wives took a cruise boat and a half load to the cobalt. Boasting an impressive striking blue hull, a foldable tower aft rumble seat, and a Volvo V8-300 HP engine with dual prop and 160 hours, this boat is k. The ergonomic helm features dual Garmin glass cockpit touchscreens, while the passenger area offers such amenities as a stowable oversize teak table and an ingenious Freezer/Fridge combo unit that gives you both functions in one space-saving package. Texas boat company's President, Bob Hammond. Its added room makes the R35 the ideal platform for a number of exciting innovations, including Helm Watch, offering passengers access to charts and cabin controls, and a space-saving, pop-up galley that deploys at the touch of a button. Great for water activities, the entire full-width swim platform lowers below the water hydraulically with the push of a button, offering easy access in or out of the water and into the boat.Ĭontinuing the Cobalt theme of luxury materials and unmistakable quality, the R35’s finely tailored upholstery covers an enormous amount of seating in an array of modern colors and textures from stem to stern. View a wide selection of Cobalt boats for sale in Lake Conroe, Montgomery, Texas, explore detailed information & find your next boat on. The largest member of the R Series, the R35 maintains the elegantly sleek lines and innovative geometry of the series while displaying a strikingly noticeable presence. ![]() Compare the signal from your unknown sample to that of the standard and estimate the concentration.This is done either directly or via the use of a capture antibody itself immobilized on the surface. Basic ELISA principles (H2) In an ELISA assay, the antigen is immobilized to a solid surface. Try several different lengths of exposure. An ELISA, like other types of immunoassays, relies on antibodies to detect a target antigen using highly specific antibody-antigen interactions. Incubate with ECL reagent for 1 min, cover with Saran wrap (remove excessive solution from the surface), and expose X-ray film in the dark room. ![]() Wash three times with TBS-T (1 x 15 min and 2 x 5 min), then once with TBS (5 min).For optimum antibody dilution, follow the manufacturer's recommendation. Incubate with secondary antibody conjugated with HRP for 30 min at room temperature.Wash three times with TBS-T (3 x 5 min).Incubate with primary antibody (0.1–10 µg/mL for purified antibody, 1:1,000–1:100,000 for antisera, 1:100–1:10,000 for hybridoma supernatant) in BSA/TBS-T for 30 min at room temperature.Alternately, you can view our other blotting protocols. Join our lab team in this western blot video protocol for expert guidance and tips. Conducting a Dot Stained test Our Dot Blot Protocol can guide i through the process with sample preparation into protein detection. Block non-specific sites by soaking in 5% BSA in TBS-T in a 10 cm Petri dish (30 min to 1 h at room temperature). Get your western blot experiment right first time with our extensive library of blotting resources: protocols, troubleshooting guides, webinars and more.Minimize the area that the solution penetrates (usually 2–4 mm diameter) by applying it slowly. Using a narrow-mouth pipette tip, spot 2 µL of sample onto the nitrocellulose membrane at the center of the grid.Draw a grid by pencil to indicate the region you are going to blot. Have the nitrocellulose membrane ready.Nitrocellulose membrane (BIO-RAD, Trans-Blot, etc.) A technique for detecting, analyzing and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.Ĭoncentration of proteins in crude preparations (such as culture supernatant) can be estimated semiquantitatively by using the dot blot method if you have both purified protein and specific antibody against it. ![]() We asked Liz Hansen, Chair of the Maine Coon Breed Committee at The International Cat Association (TICA), about the origins of the breed: "There are a few different theories of the origin of the Maine Coon. In domestic species, size is also (increasingly) down to strategic breeding, with particularly hefty specimens being favoured in order to normalize the trait of largeness across the breed. Of course, it’s not purely down to Mother Nature. It’s no coincidence that many of the largest cat breeds – such as Maine Coons from the most north-eastern US state and Norwegian forest cats from northern Europe – also sport long, thick fur for extra insulation. Animals essentially evolve to be bigger in order to decrease their surface area relative to volume, thereby reducing heat loss in cold-climate habitats. ![]() According to Bergmann’s rule, warm-blooded animals of the same species (in this case, Felis catus) generally increase in size at higher latitudes, as temperatures drop.Īlso known as the square-cube principle, it reflects the ratio between bodily surface area and volume. Many think that it’s down to the part of the world that they originate. So why do certain breeds grow so much bigger than others and become the largest domestic cats? Other large cat breeds are ragdolls, Birmans and Norwegian forest cats – the latter of which are very similar in appearance to Maine Coons, so it's likely that the two share a genetic heritage. As you’ve probably already guessed from their name, their origins lie in Maine, USA, where they have been the official state cat since 1985. In the cat world, Maine Coons are well-known for being "gentle giants", typically reaching 76–101 cm (30–40 in) long and standing up to 40 cm (16 in) high, compared to 23–25 cm (9–10 in) tall for most house cats. This particular breed – also known as the American longhair – has held the title of Longest domestic cat for almost a decade. In fact, he's a bit of a local celebrity as the couple sometimes take their pet out for a stroll in a buggy – a sight you’re not likely to quickly forget!īarivel has something of a biological advantage when it comes to this record: he’s a Maine Coon cat. ![]() To put that in context, that’s longer than a baseball bat or equivalent to seven iPads laid out in a row!īased in Vigevano, near Milan, this full-stretch feline lives with his owners Cinzia Tinnirello (below) and Edgar Scandurra (both Italy). When measured from head to tail tip on, Barivel came in at 120 cm (3 ft 11 in). This "grande gatto" is the latest inductee to the hallowed hall of Longest domestic cats. In case you were wondering, Himmy was noted to be 96 cm (3 ft 1 in) in length, so would have fallen some way short of the recently announced new holder (below). However, GWR no longer recognizes weight as a measurement for domesticated animals, focusing instead on length and height. For decades, the Largest domestic cat was a tabby called Himmy from Queensland, Australia, who weighed in at 21.3 kg (46 lb 15 oz) when he died in 1986. Historically, GWR focused more on the weight of a cat to determine which was the biggest. The debut holder of the title was a four-year-old cat called Snowbie from Scotland, UK, who was listed as 103 cm (3 ft 4 in). Over the years, Guinness World Records (GWR) has had the honour of meeting a wide variety of fantastic felines from the world's Oldest cat, to the Furriest to the cat with the Longest whiskers… (Here’s a small selection that we celebrated on 2017’s International Cat Day.)īut some of the most memorable moggies ever to have graced the pages of Guinness World Records have to be the Longest and Tallest domestic cats, one of which we’re excited to say has a new holder…Īs with several GWR pet records, categorizing size by length is a relatively "new" way of doing things, with the first documented Longest domestic cat dating to the late 1990s. ![]() The Captain will unlock when you will beat Mithrix, the final boss. With a little practice, you can use the Captain perfectly and kill any enemy without any difficulty. All becomes possible due to his effective defense and strong destructive potential. But if you get him once, you can tackle all the situations with ease. The captain is the most challenging character to unlock. A 5% chance of attaching sticky bombs on each stack can take your performance to another level. Sticky Bombs: With sticky bombs, you can deal 180% damage to the enemies.Playing him is great fun because of his efficient optimization of the items. Playstyle: Though the Captain is not that easy to unlock but once got, you can destroy the enemy troops within no time.2- Captain Captain Captain’s Stats Health Stun Grenade works quite well with the Suppressive Fire for keeping the threads passive until you are able to kill them. The builds you must strive for are critical hit chance, attack speed, Ukulele lightning, Gasoline, and Kjaro’s band. If you are a beginner, focus on those items that can provide you with more DPS (Damage Per Second). If we talk about its builds, they make the commando to move in different directions conveniently. All other characters outdo Commando, so when you unlock them, there is no reason to use them. Moreover, the worst utility skill also makes it the weakest character of this game. The area of effect abilities are a must in Risk of Rain 2 but Commando has no excel to them. But the problem is that its abilities are too limited and bland. Its playstyle is straightforward You can aim and shoot the opponents and use the abilities it comes with. A perfect item for double-tap shots.Ĭommando is the first character that you have access to when start playing the game. Gasoline: What an enormous item it is that burns the combatants and as they vanish, it spreads widely.Offering moderate firepower which can penetrate through the opponents to keep you going. Playstyle: It’s the best character for both team play and solo play because it is a well-balanced character.Phase Round (The piercing bullet will hit the entire enemies standing in a line with 230% damage) 1- Commando Commando Commando’s Statsĭouble Shoot (Double shoot to get 2×90% damage) All Items are also mentioned, and you can go through our Item Tier list to get additional data. In this section, we are talking about different popular characters of Risk of Rain 2 and the specific builds required for them. Interested in getting more Risk of Rain 2 achievement, check our guide here. Also, If you haven’t unlocked all these characters, go through our Risk of Rain 2 Character Unlock guide as well. Let’s talk about the different Risk of Rain 2 builds you can create conveniently. The builds will give you extra strength and power to survive in the game. It includes 12 playable characters and each character comes with multiple skills, stats, attacks, strategies, and builds. ![]() ![]() It consists of a wide variety of playstyles, weapons, progression systems, and enemies. Every moment of your game becomes exciting as it provides 100 different items which make each run distinctive. Risk of Rain 2 is a supremely action-packed rogue-like game that is an upgraded version offering tremendous features than its predecessor.
![]() 33 odrive Mountain Duck odrive aggregates all cloud storage. Access, sync, share, and encrypt everything in one place. ![]() Mountain Duck lets you mount server and cloud storage as a disk in Finder on macOS and the File Explorer on Windows. Mountain Duck lets you mount server and cloud storage as a disk on your desktop.Additionally, you can apply this principle to all of your bookmarks and not only on the already mentioned cloud storage provider. Open Mountain Duck and connect to the desired Bookmark (We’ll use Dropbox in our example). With Smart Synchronization, files are synchronized to the local disk when opened to allow offline usage and changes are uploaded in the background as soon as a connection is available. Right-click on the desired file or folder and choose Sync Options → Keep Offline on Local Disk in the context menu.Navigate to the file or folder that you want to keep on the disk for offline usage.Open remote files with any application and work like on a local volume. If you support or use a file lock, the file locked on the WebDAV server. If you are using macOS you have to activate the Finder Extension in order to reach the described option. Wait until the file is successfully synchronized.Access Dropbox without having to sync your documents to your local drive. The chosen file or folder should show the following badge icon.Mountain Duck 2.0 opens by promising "foolproof" client-side encryption for cloud storage, allowing users to add another layer of encryption to their cloud-hosted data using Cryptomator, which provides AES encryption and scrypt-powered protection against brute force attacks. You can right-click any file or folder and select Mountain Duck to access useful features such as copying the URL, downloading files, reloading them, etc. Supported services include Dropbox, Google Drive, OneDrive, Microsoft Azure and Amazon S3, along with OpenStack Swift, WebDAV and FTP hosting. The app costs US39 (A53) and supports multiple cloud storage services, including Google Drive and OneDrive. You can check out its features for free with a two-week trial. Users can even use the service to browse and encrypt folders stored locally. The feature is made simple by users simply right-clicking a folder and choosing 'New Encrypted Vault' before providing a passphrase (Mac users must first enable the Mountain Duck extensions via System Preferences > Extension > Finder).Īlso new in version 2.0 is an Info panel, which is also accessed through the context menu. From here users gain service-specific features such as changing permissions for files on FTP or SFTP, ACLs on Amazon S3, Microsoft Azure and Backblaze B2, and changing metadata on selected services. Windows users also gain the ability to register Mountain Duck as the protocol handler for sftp://, ftp://, ftps://, dav://, davs:// and s3:// schemes. Mountain Duck 2.0 is available as a free trial download for PC (Windows Vista or later) or Mac (OS X 10.8.3 or later). ![]() ![]() Examples include Expandrive, Mountain Duck and others. The full version costs $39 for one user, with upgrades from previous versions starting from 40 percent and rising to 100 percent depending on date of purchase. However, if a storage service can search, analyze or index your files for you, guess what they. ![]() “You’d think it’d be impossible for any writer to put together a Roger Corman biography that's anywhere near as fun as his movies, but Entertainment Weekly writer/critic Chris Nashawaty has done just that. “Author Nashawaty deftly describes how Corman’s legacy is far more nuanced than most realize.” - American Way magazine “It includes in-depth aesthetic appreciations of ten of Corman’s movies, which, taken together, make a compelling case for Corman as an artist.”. “An enthusiastic ode to colorful, seat-of-your-pants filmmaking, this one’s hard to beat.” - Booklist (starred review) “This new coffee table book, brimming with outrageous stills from many of Corman’s hundreds of films, looks at the wild career of the starmaker who was largely responsible for so much of the Hollywood we know today.” - New York Post Directed by Ivan Reitman Writing Credits Cast (in credits order) verified as complete Produced by Music by Elmer Bernstein Cinematography by Bill Butler. Praise for Crab Monsters, Teenage Cavemen, and Candy Stripe Nurses: ![]() Despite being a broad farce with implausible situations, the. ![]() As told by Corman himself and graduates of “The Corman Film School,” including Peter Bogdanovich, James Cameron, Francis Ford Coppola, Robert De Niro, and Martin Scorsese, this comprehensive oral history takes readers behind the scenes of more than six decades of American cinema, as now-legendary directors and actors candidly unspool recollections of working with Corman, continually one-upping one another with tales of the years before their big breaks.Ĭrab Monsters is supplemented with dozens of full-color reproductions of classic Corman movie posters behind-the-scenes photographs and ephemera (many taken from Corman’s personal archive) and critical essays on Corman’s most daring films-including The Intruder, Little Shop of Horrors, and The Big Doll House- that make the case for Corman as an artist like no other. Stripes (1981) Stripes (1981) Stripes is a 1981 military comedy starring Bill Murray and Harold Ramis (both would later re-team with director Ivan Reitman for the 1984 comedy classic Ghostbusters) as two buddies who decide to leave their crumbling civilian lives and join the US Army. C rab Monsters, Teenage Cavemen, and Candy Stripe Nurses is an outrageously rollicking account of the life and career of Roger Corman-one of the most prolific and successful independent producers, directors, and writers of all time, and self-proclaimed king of the B movie. In a ’70s world where it was by then well understood that a saloon could be made to handle as well as – perhaps even better than – a sports car, then a saloon-based coupé such as the original Manta couldn’t be a bad thing.Īnd if rare, luxurious and expensively engineered coupés were the birthright of names such as Lancia, Alfa and BMW, then Opel saw no reason why it shouldn’t have a piece of the action by selling the same dream at a price your parents could afford. ![]() The Stingray-like pointed roof with its louvers was replaced with a long, pointed, scooped out design that was very cool. Opel’s rallying prowess is steeped in the Ascona and Manta ‘B’, but you still sense the link to those cars in the Manta ‘A’, which proved that there was plenty of life left in traditionally contrived underpinnings. The covers for the side pipes eventually showed up as an option on the ‘69 Corvette Most dramatic was the all-new roof line. ![]() They are agile and inherently safe, and offer no tricks to catch out the unwary. While on a fishing excursion, Mitchell was inspired by the manta Ray powerfully moving through the ocean, which inspired the Corvette Mako Shark II/Manta. ![]() Both Mantas understeer just enough to maintain your confidence, with firm damping to help hold your line through steering that is more responsive than its 4.1 turns lock-to-lock would suggest. Racecar driver and car tuner and entrepreneur Angie Mead King recently posted her latest project, the Black Manta at the 2016 SEMA Show in Las Vegas. |
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