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![]() To learn more about how and for what purposes Amazon uses personal information (such as Amazon Store order history), please visit our Privacy Notice. Clear some space in your living room, your Star Wars action figures will be dwarfed by this life-size Han. Please see the certificate pictured below for more information about the replica and its creation.Height: 80Width: 32Depth: 14Weight: 100 poundsShipping will. Made of durable foam, this Star Wars Han Solo in Carbonite Life-Size Foam Statue measures just over 7-feet tall, features hand-painted details, and includes light-up features in its base. While searching for a review on the Rubies Han Solo in Carbonite foam replica, I found this thread among others. You can change your choices at any time by visiting Cookie Preferences, as described in the Cookie Notice. Buy a full-size Han Solo in carbonite for 7,000. OFFICIALLY LICENSED STAR WARS Han Solo in Carbonite Statue LIFE-SIZE statue standing over 7 feet tall high-density polyurethane foam movie replica with light up base. Click ‘Customise Cookies’ to decline these cookies, make more detailed choices, or learn more. Third parties use cookies for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products. This includes using first- and third-party cookies, which store or access standard device information such as a unique identifier. If you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie Notice. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. DOWN TO THE NITTY GRITTY, DON'T MISS OUT ONLY TWO LEFT. Price: 248.44 for 24 months with PayPal Credit Add to Watchlist. Star Wars Han Solo Carbonite Life Size Statue w/ Lights Limited Edition Prop. We use cookies and similar tools that are necessary to enable you to make purchases, to enhance your shopping experiences and to provide our services, as detailed in our Cookie Notice. Star Wars Han Solo In Carbonite Life Size Statue Limited Edition Disney LM Treasures - R 4,500.00 SKU: LP-69081-R Out Of Stock To Pre-Order Contact (626) 252-7354 Notify me via: Sms Notify Me ITEM NO LONGER BEING PRODUCED AND IS DISCONTINUED. Star Wars Han Solo Carbonite Life Size Statue w/ Lights Limited Edition Prop. ![]() Choose fieldsĪlthough a suggester has several properties, it's primarily a collection of string fields for which you're enabling a search-as-you-type experience. For more information, see How to rebuild an Azure Cognitive Search index. Given that existing fields are already tokenized, you'll have to rebuild the index if you want to add them to a suggester. Prefixes are generated during indexing, when partial terms in two or more character combinations are tokenized alongside whole terms. If you try to create a suggester using pre-existing fields, the API will disallow it. Use the default standard Lucene analyzer ( "analyzer": null) or a language analyzer (for example, "analyzer": "en.Microsoft") on the field. If the string field is part of a complex type (for example, a City field within Address), include the parent in the field path: "Address/City" (REST and C# and Python), or (JavaScript). The best time to create a suggester is when you're also defining the field that will use it. A suggester takes a name and a collection of fields over which the typeahead experience is enabled. To create a suggester, add one to an index definition. Both requests target the documents collection of specific index and responses are returned after a user has provided at least a three character input string. You can implement both typeahead behaviors within the same search solution if you want an experience similar to the one indicated in the screenshot. Search-as-you-type support is enabled on a per-field basis for string fields. The remainder of this article is focused on creating a suggester.Ĭall a suggester-enabled query, in the form of a Suggestion request or Autocomplete request, using one of the APIs listed below. To implement these behaviors in Azure Cognitive Search, there's an index and query component.Īdd a suggester to a search index definition. You can use these features separately or together. For suggestions, you can surface any field that provides descriptive information. Suggestions are mini search results, where a field like hotel name represents a matching hotel search document from the index. Autocomplete anticipates a potential term, finishing "tw" with "in". The following screenshot from Create your first app in C# illustrates both. Typeahead can be autocomplete, which completes a partial input for a whole term query, or suggestions that invite click through to a particular match. ![]() Typeahead experiences in Cognitive Search The same suggester supports both experiences. Matches on partial terms can be either an autocompleted query or a suggested match. For example, a suggester that includes a City field with a value for "Seattle" will have prefix combinations of "sea", "seat", "seatt", and "seattl" to support typeahead. ![]() A suggester provides a list of fields that undergo extra tokenization, generating prefix sequences to support matches on partial terms. In Azure Cognitive Search, typeahead or "search-as-you-type" is enabled through a suggester. Protein carbonylation is the most commonly used measure of oxidative modification of proteins. Quantitation of protein carbonylation by dot blot. This level of sensitivity allowed measurement of protein carbonylation in individual Drosophila. Sixty ng protein is sufficient to measure protein carbonyl content. The detection limit is 0.19 ± 0.04 pmol carbonyl. It also readily dissolves 2,4-dinitrophenylhydrazine and wets PVDF membranes. Dimethyl sulfoxide was employed as the solvent because it very efficiently extracts proteins from tissues and keeps them soluble. We developed an immunochemical dot blot method for quantitation of protein carbonylation in homogenates or purified proteins. It is frequently measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent, 2,4-dinitrophenylhydrazine. Quantitation of Protein Carbonylation by Dot Blot The 5-mC dot blot is a reliable, simple, and rapid method to detect the general DNA methylation level to evaluate chondrocyte phenotype. We found that the DNA methylation level differed between the monolayer subcultures, and therefore could play a key role in chondrocyte dedifferentiation. In addition, dot blots can detect overall DNA methylation level using a commercially available 5-mC antibody. Compared with other gel electrophoresis-based blotting approaches and other complex blotting procedures, the dot blot method saves significant time. In this dot blot approach, a gDNA mixture containing the methylated DNA to be detected was spotted directly onto an N + membrane as a dot inside a previously drawn circular template pattern. The gDNA methylation level was detected using a methylation-specific dot blot assay. In this study, genomic DNA (gDNA) was extracted from human chondrocytes cultured with varying number of passages. Additionally, the prohibitively high cost of HPLC instruments limits HPLC's wider application. However, HPLC requires complete digestion of genomic DNA. Other methods used to quantify global DNA methylation levels include high-performance liquid chromatography (HPLC). Due to DNA degradation during bisulfite conversion, these methods typically require a large sample volume. Current genome-wide methylation analysis techniques largely rely on bisulfite genomic sequencing. Therefore, it is important to establish a precise, simple, and rapid method to quantify global DNA methylation levels during chondrocyte dedifferentiation. However, results based on different experimental methods can be inconsistent. The global DNA methylation level of chondrocytes is considered to be a suitable biomarker for the loss of the chondrocyte phenotype. The dedifferentiation of hyaline chondrocytes into fibroblastic chondrocytes often accompanies monolayer expansion of chondrocytes in vitro. Jia, Zhaofeng Liang, Yujie Ma, Bin Xu, Xiao Xiong, Jianyi Duan, Li Wang, Daping All rights reserved.Ī 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro. This article describes details of the development of an automated identity assay for a 15-valent pneumococcal conjugate vaccine, PCV15-CRM197, using capillary Western technology. An advantage this capillary Western technique has over the traditional manual dot blot Western method is the speed and the automation of electrophoresis separation, blotting, and detection steps performed in 96 capillaries. Both techniques are usually very tedious, labor-intensive, and complicated for multivalent vaccines, and they can be difficult to transfer to other laboratories. Traditionally, identity assays for biological products are performed using either an enzyme-linked immunosorbent assay (ELISA) or a manual dot blot Western. Simple Western is a new technology that allows for the separation, blotting, and detection of proteins similar to a traditional Western except in a capillary format. Hamm, Melissa Ha, Sha Rustandi, Richard R Automated capillary Western dot blot method for the identity of a 15-valent pneumococcal conjugate vaccine. ![]() Here's the ingredients label for a bottle of Jose Cuervo Classic Margarita Mix (Non-Alcoholic) :ĭepending on which version you buy, it may have high fructose corn syrup (HFCS) or sucralose instead. Now that you know what's supposed to go in a margarita, let's take a look at a popular store-bought Margarita Mix. To make it simple, it's mostly tequila and citrus with a bit of sugar and depending on the person's preference, salt on the rim. There are lots of ways to make a margarita, but a classic version generally has tequila, lime juice, an orange liqueur (like triple sec, Cointreau or Grand Marnier) or orange juice, and a sweetener like simple syrup to balance out the lime's tartness. Unfortunately, these margarita mixes are filled with crappy, fake ingredients. ![]() But I like real juices and mixers in my drinks, as should most people. Cause those mixes are d-i-s-g-u-s-t-i-n-g. :) Second, I know WAY too many people out there are still drinking pre-made mixes and this makes me very sad. I experimented with many cocktails and martinis during my college years. Lime Wedges – A must for garnish, and also to help the salt stick to the rim.I am SO excited to share this margarita recipe with you guys! Know why? First, I concocted it several years ago when I was living in KC with my sister and attending UMKC.Coarse sea salt works very nicely, but any kind you have is fine! Sea Salt – For salting the rim of your margarita glasses.And unlike stevia that I used to use, Besti doesn’t have that stevia aftertaste. I have tried making this drink with erythritol before, which works okay, but doesn’t dissolve very well. Sweetener – Most skinny margarita recipes are really just their regular counterparts in disguise, using agave nectar… which is still sugar! I use Besti Powdered Monk Fruit Allulose Blend in mine and it tastes the same without all the sugar! Unlike other monk fruit sweeteners that contain erythritol, this one dissolves easily in cold liquids. ![]() FYI, Triple Sec is an orange liqueur and contains 11 grams of sugar per ounce.
![]() Monza, Silverstone, Imola, Mugello, Spa, Brands Hatch and many more tracks, including the historical reproduction of Monza, that brings again to life the legendary Sopraelevata, the high-speed oval where races were staged until 1961. The circuits have been developed using Laser scan technology, in order to guarantee the highest level of accuracy possible in reproducing real world motor racing environments. Extremely detailed with single player and multiplayer options, exclusive licensed cars reproduced with the best accuracy possible, thanks to the official cooperation of Car Manufacturers.ĪSSETTO CORSA has been developed at the KUNOS Simulazioni R&D office, located just inside the international racing circuit of Vallelunga, allowing the team to develop the game with the cooperation of real world racing drivers and racing teams. The advanced physics engine is being designed to provide a very realistic driving experience, including features and aspects of real cars, never seen on any other racing simulator such as tyre flat spots, heat cycles including graining and blistering, very advanced aerodynamic simulation with active movable aerodynamics parts controlled in real time by telemetry input channels, hybrid systems with kers and energy recovery simulation. Assetto Corsa features an advanced DirectX 11 graphics engine that recreates an immersive environment, dynamic lighthing and realistic materials and surfaces. The psychiatric unit is an example of a subpart that could have its own NPI if the hospital determines that it should. (1) The psychiatric unit in a hospital is not a legal entity but is part of the hospital (the "parent"), which is a legal entity. Here are three examples of organization health care providers that may be considered subparts and may apply for NPIs if so directed by their "parents": Many organization health care providers who apply for NPIs are not legal entities themselves but are parts of other organization health care providers that are legal entities (the "parents"). The Parent Organization LBN and TIN fields can only be completed if the answer to the subpart question is Yes. If the organization is a subpart =, the Parent Organization Legal Business Name (LBN) and Parent Organization Taxpayer Identification Number (TIN) fields must be completed. The "Is the organization a subpart?" question must be answered. 2 = (Non-person): entity other than an individual human being that furnishes health care (for example, hospital, SNF, hospital subunit, pharmacy, or HMO).1 = (Person): individual human being who furnishes health care. ![]() ![]() Full Replica of the CMS (NPPES) NPI Record Field Nameġ0-position all-numeric identification number assigned by the NPS to uniquely identify a health care provider.Ĭode describing the type of health care provider that is being assigned an NPI. This thank-yousir to Glastron dealers and their wives took a cruise boat and a half load to the cobalt. Boasting an impressive striking blue hull, a foldable tower aft rumble seat, and a Volvo V8-300 HP engine with dual prop and 160 hours, this boat is k. The ergonomic helm features dual Garmin glass cockpit touchscreens, while the passenger area offers such amenities as a stowable oversize teak table and an ingenious Freezer/Fridge combo unit that gives you both functions in one space-saving package. Texas boat company's President, Bob Hammond. Its added room makes the R35 the ideal platform for a number of exciting innovations, including Helm Watch, offering passengers access to charts and cabin controls, and a space-saving, pop-up galley that deploys at the touch of a button. Great for water activities, the entire full-width swim platform lowers below the water hydraulically with the push of a button, offering easy access in or out of the water and into the boat.Ĭontinuing the Cobalt theme of luxury materials and unmistakable quality, the R35’s finely tailored upholstery covers an enormous amount of seating in an array of modern colors and textures from stem to stern. View a wide selection of Cobalt boats for sale in Lake Conroe, Montgomery, Texas, explore detailed information & find your next boat on. The largest member of the R Series, the R35 maintains the elegantly sleek lines and innovative geometry of the series while displaying a strikingly noticeable presence. ![]() Compare the signal from your unknown sample to that of the standard and estimate the concentration.This is done either directly or via the use of a capture antibody itself immobilized on the surface. Basic ELISA principles (H2) In an ELISA assay, the antigen is immobilized to a solid surface. Try several different lengths of exposure. An ELISA, like other types of immunoassays, relies on antibodies to detect a target antigen using highly specific antibody-antigen interactions. Incubate with ECL reagent for 1 min, cover with Saran wrap (remove excessive solution from the surface), and expose X-ray film in the dark room. ![]() Wash three times with TBS-T (1 x 15 min and 2 x 5 min), then once with TBS (5 min).For optimum antibody dilution, follow the manufacturer's recommendation. Incubate with secondary antibody conjugated with HRP for 30 min at room temperature.Wash three times with TBS-T (3 x 5 min).Incubate with primary antibody (0.1–10 µg/mL for purified antibody, 1:1,000–1:100,000 for antisera, 1:100–1:10,000 for hybridoma supernatant) in BSA/TBS-T for 30 min at room temperature.Alternately, you can view our other blotting protocols. Join our lab team in this western blot video protocol for expert guidance and tips. Conducting a Dot Stained test Our Dot Blot Protocol can guide i through the process with sample preparation into protein detection. Block non-specific sites by soaking in 5% BSA in TBS-T in a 10 cm Petri dish (30 min to 1 h at room temperature). Get your western blot experiment right first time with our extensive library of blotting resources: protocols, troubleshooting guides, webinars and more.Minimize the area that the solution penetrates (usually 2–4 mm diameter) by applying it slowly. Using a narrow-mouth pipette tip, spot 2 µL of sample onto the nitrocellulose membrane at the center of the grid.Draw a grid by pencil to indicate the region you are going to blot. Have the nitrocellulose membrane ready.Nitrocellulose membrane (BIO-RAD, Trans-Blot, etc.) A technique for detecting, analyzing and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.Ĭoncentration of proteins in crude preparations (such as culture supernatant) can be estimated semiquantitatively by using the dot blot method if you have both purified protein and specific antibody against it. ![]() We asked Liz Hansen, Chair of the Maine Coon Breed Committee at The International Cat Association (TICA), about the origins of the breed: "There are a few different theories of the origin of the Maine Coon. In domestic species, size is also (increasingly) down to strategic breeding, with particularly hefty specimens being favoured in order to normalize the trait of largeness across the breed. Of course, it’s not purely down to Mother Nature. It’s no coincidence that many of the largest cat breeds – such as Maine Coons from the most north-eastern US state and Norwegian forest cats from northern Europe – also sport long, thick fur for extra insulation. Animals essentially evolve to be bigger in order to decrease their surface area relative to volume, thereby reducing heat loss in cold-climate habitats. ![]() According to Bergmann’s rule, warm-blooded animals of the same species (in this case, Felis catus) generally increase in size at higher latitudes, as temperatures drop.Īlso known as the square-cube principle, it reflects the ratio between bodily surface area and volume. Many think that it’s down to the part of the world that they originate. So why do certain breeds grow so much bigger than others and become the largest domestic cats? Other large cat breeds are ragdolls, Birmans and Norwegian forest cats – the latter of which are very similar in appearance to Maine Coons, so it's likely that the two share a genetic heritage. As you’ve probably already guessed from their name, their origins lie in Maine, USA, where they have been the official state cat since 1985. In the cat world, Maine Coons are well-known for being "gentle giants", typically reaching 76–101 cm (30–40 in) long and standing up to 40 cm (16 in) high, compared to 23–25 cm (9–10 in) tall for most house cats. This particular breed – also known as the American longhair – has held the title of Longest domestic cat for almost a decade. In fact, he's a bit of a local celebrity as the couple sometimes take their pet out for a stroll in a buggy – a sight you’re not likely to quickly forget!īarivel has something of a biological advantage when it comes to this record: he’s a Maine Coon cat. ![]() To put that in context, that’s longer than a baseball bat or equivalent to seven iPads laid out in a row!īased in Vigevano, near Milan, this full-stretch feline lives with his owners Cinzia Tinnirello (below) and Edgar Scandurra (both Italy). When measured from head to tail tip on, Barivel came in at 120 cm (3 ft 11 in). This "grande gatto" is the latest inductee to the hallowed hall of Longest domestic cats. In case you were wondering, Himmy was noted to be 96 cm (3 ft 1 in) in length, so would have fallen some way short of the recently announced new holder (below). However, GWR no longer recognizes weight as a measurement for domesticated animals, focusing instead on length and height. For decades, the Largest domestic cat was a tabby called Himmy from Queensland, Australia, who weighed in at 21.3 kg (46 lb 15 oz) when he died in 1986. Historically, GWR focused more on the weight of a cat to determine which was the biggest. The debut holder of the title was a four-year-old cat called Snowbie from Scotland, UK, who was listed as 103 cm (3 ft 4 in). Over the years, Guinness World Records (GWR) has had the honour of meeting a wide variety of fantastic felines from the world's Oldest cat, to the Furriest to the cat with the Longest whiskers… (Here’s a small selection that we celebrated on 2017’s International Cat Day.)īut some of the most memorable moggies ever to have graced the pages of Guinness World Records have to be the Longest and Tallest domestic cats, one of which we’re excited to say has a new holder…Īs with several GWR pet records, categorizing size by length is a relatively "new" way of doing things, with the first documented Longest domestic cat dating to the late 1990s. |
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